Introgression of anthracnose resistance Co-4 gene in elite French bean (Phaseolus vulgaris L.) background using linked molecular marker SY20

French bean is one of the most important staple foods in many parts world. Among several bacterial, fungal and viral diseases, anthracnose caused by Colletotrichum lindemuthianum widespread severe disease bean. Therefore, present study was undertaken as prebreeding effort with objective introgression resistance gene Co-4 elite cultivar. Hybridization between resistant genotype, TO having cultivar Arka Komal done. Cross × further advanced to generate, F1, BC1F1, BC2F1 BC2F2 plants. Testing hybridity resultant plants/pods using linked SCAR marker SY20 resulted identification 46 gene-positive BC2F2. Further foreground selection revealed lack segregation for target 16 progenies. Screening these homozygous progenies (Co-4 gene) following detached pod germinated seed dip methods race 3 C. validated imparted all BC2F3 Agronomic evaluation BC2F4 8 qualitative traits 9 quantitative led AKTO 4, 5, 7 43 better background. These can either be used donors pyramiding or backcrossing develop essentially derived variety parent, Komal.